Journal article
YBX1/YB-1 induces partial EMT and tumourigenicity through secretion of angiogenic factors into the extracellular microenvironment
SK Gopal, DW Greening, RA Mathias, H Ji, A Rai, M Chen, HJ Zhu, RJ Simpson
Oncotarget | IMPACT JOURNALS LLC | Published : 2015
Abstract
Epithelial-mesenchymal transition (EMT) describes a morphogenetic program which confers mesenchymal cell properties, such as reduced cell-cell contact and increased cell migration and invasion, to epithelial cells. Here we investigate the role of the pleiotropic transcription/splicing factor and RNA-binding protein nucleasesensitive element-binding protein 1 (YBX1/YB-1) in increasing the oncogenic potential of epithelial MDCK cells. Characterization of MDCK cells expressing YBX1 (MDCKYBX1 cells) revealed a partial EMT phenotype, including cytosolic relocalization of E-cadherin, increased cell scattering, and anchorage-independent growth. Subcutaneous injection of parental MDCK cells into NOD..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors are supported, in part, by the National Health and Medical Research Council of Australia program grant 487922 (R.J.S.), project grant 1057741 (R.J.S), 628727 (HJ.Z), Melbourne Research Grant Support Scheme (Melbourne University, HJ.Z) and Early Career CJ Martin Fellowship APP1037043 (R.A.M.). S.K.G/A.R/M.C are supported by La Trobe University Postgraduate Scholarships. The authors would like to thank Dr. Jacqueline Orian for assistance with immunohistochemistry. Proteomics in this study was supported using the LIMS Mass Spectrometry and Proteomics facility.